The Polymerase Chain Reaction, PCR, is one of the cornerstones of molecular biology. It allows the selective amplification of a target DNA sequence that occurs only once within a large and complex DNA structure such as genomic DNA. PCR is composed
Cloning is a ubiquitous multi-step technique in molecular biology labs. We have previously discussed restriction digestion and ligation, so it’s time to conclude with transformation and colony screening.
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